clustal w program  (DNASTAR)

Journal: Pesticide Biochemistry and Physiology

Article Title: Development and efficacy of dsRNA pesticides targeting the Colorado potato beetle with enhanced stability via chitosan formulations

doi: 10.1016/j.pestbp.2025.106606

Figure Lengend Snippet: Fig. 1. Molecular characterization of CPB proteasome subunit beta type-5 (CPB-PSMB5). (A) Prediction of signature motifs and alignment of nucleotide sequences of CPB-PSMB5 with other four well-known insect and potato plant PSMB5. Sequence alignment used Clustal W program of MegAlign (DNASTAR, Version 7.0). Species acronyms and GenBank accession numbers of PSMB5 amino acids are listed in Table S1. (B) Predicted three-dimensional structure of CPB-PSMB5 showing key structural domains. Alpha-helices and beta-sheets are depicted in the model as cyan, and yellow, respectively. The beta subunit interaction site domain is highlighted in brown. Green regions represent the active site. (C) Phylogenetic tree of CPB-PSMB5 (highlighted in a red rounded rectangle) and other PSMB5 sequences from different insect orders. The tree was generated with MEGA 7 using Neighbor-joining method. Bootstrap values on nodes were obtained with 1000 replicates. GenBank accession numbers are provided in Supplementary Table S1. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Sequence alignment was performed using the Clustal W program within MegAlign (DNASTAR, Version 7.0).

Techniques: Sequencing, Generated